Genome‐scale molecular principles of mRNA half‐life regulation in yeast

Precise control of protein and messenger RNA (mRNA) degradation is essential for cellular metabolism homeostasis. Controlled specific both molecular species necessitates their engagements with the respective machineries; this engagement involves a disordered/unstructured segment substrate traversing tunnel machinery accessing catalytic sites. However, while factors influencing have been extensively explored on genome scale, in multiple organisms, such comprehensive understanding remains missing mRNAs. Here, we analyzed genome-scale experimental yeast mRNA half-life data light experimentally derived secondary structures binding data, along high-resolution X-ray crystallographic RNase machines. Results unraveled consistent trend that mRNAs comprising longer terminal and/or internal unstructured segments significantly shorter half-lives; lengths 5′-terminal, 3′-terminal, affect are compatible 5′ exo-, 3′ endoribonuclease machineries. Sequestration into ribonucleoprotein complexes elongates half-life, presumably by burying ribonuclease sites under oligomeric interfaces. After gene duplication, differences lengths, proportions segments, oligomerization modes result altered half-lives paralogous Side-by-side comparison principles underlying controlled unravels remarkable mechanistic similarities suggests how intrinsic structural features two species, at different levels central dogma, regulate scale.