Globin Gene Switching in Transgenic Mice Carrying HS2-Globin Gene Constructs

Globin gene switching in transgenic mice carrying HS2-globin gene constructs.

We have examined the pattern of human globin gene switching in transgenic mice containing three different gamma and beta gene constructs (HS2G gamma A gamma delta beta, HS2A gamma beta neo, and HS2A gamma en beta) and compared the results with previously described transgenics (HS2A gamma beta, HS2G gamma A gamma-117 delta beta, and LCR epsilon G gamma A gamma delta beta). Developmental regulati...

Analysis of the Human {-Globin Gene Promoter in Transgenic Mice

{-Globin is the embryonic form of the O( chain of hemoglobin. Transgenic mice generated with {-globin constructs containing the {-globin gene, 557 bp of 5 flanking sequence, and 2-kb of 3 flanking sequence linked to the @globin locus control region hypersensitive site 2 (HS2) expressed human {-globin only in embryonic yolk sac erythroid tissue, and not in definitive erythroid tissue in the feta...

g-Globin Promoter Prevents Silencing of a Linked Human b-Globin Gene in Transgenic Mice

Acetylation of a Specific Promoter Nucleosome Accompanies Activation of the ε-Globin Gene by b-Globin Locus Control Region HS2

DNA methylation: a secondary event in globin gene switching?

Changes in gamma-globin gene methylation accompany the fetal to adult globin switch in man. Using somatic cell hybrids made by fusing mouse erythroleukemia and human fetal erythroid cells, we asked whether methylation is a cause or a consequence of gamma-gene inactivation during development. These hybrids initially express human gamma-globin but switch with time in culture to adult globin gene ...