Gradient field microscopy of unstained specimens
نویسندگان
چکیده
منابع مشابه
Gradient field microscopy of unstained specimens
We present a phase derivative microscopy technique referred to as gradient field microscopy (GFM), which provides the first-order derivatives of the phase associated with an optical field passing through a transparent specimen. GFM utilizes spatial light modulation at the Fourier plane of a bright field microscope to optically obtain the derivatives of the phase and increase the contrast of the...
متن کاملGradient field microscopy of unstained specimens: reply to comment.
We reply to the comment written by Ferrari and Ayubi on our recent paper, Kim et al. [Opt. Express, 20, 6737 (2012)]. We maintain that our use of Fourier filtering methods lead to valuable intrinsic contrast live cell imaging. Judging by their comments regarding the limit sin(x)~x, it seems that the authors overlooked Fig. 2 in our paper, where we show results with sinusoidal masks of different...
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The phase shift of light or electrons in objects is now necessary for probing weak-phase objects such as unstained biological specimens. Optical microscopy (OM) and transmission electron microscopy (TEM) have been used to observe weak-phase objects. However, conventional OM has low spatial resolution and TEM is limited to thin specimens. Here, we report on the development of dark-field X-ray pt...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2012
ISSN: 1094-4087
DOI: 10.1364/oe.20.006737