Harvesting microalgae by CTAB-aided foam flotation increases lipid recovery and improves fatty acid methyl ester characteristics

Effective harvesting of microalgae by coagulation–flotation

This study developed a coagulation-flotation process for microalgae Chlorella sp. XJ-445 harvesting, which was composed of algal surface modification by combined use of Al3+ and cetyltrimethylammonium bromide (CTAB) and followed dispersed bubble flotation. Dissolved organic matter (DOM) in the medium was firstly characterized and mainly consisted of hydrophilic low molecular weight molecules. T...

FATTY ACID METHYL ESTER PROFILING OF Enterococcus and E

Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae.

Fatty acid ethyl esters (FAEEs) are a form of biodiesel that can be microbially produced via a transesterification reaction of fatty acids with ethanol. The titer of microbially produced FAEEs can be greatly reduced by unbalanced metabolism and an insufficient supply of fatty acids, resulting in a commercially inviable process. Here, we report on a pathway engineering strategy in Saccharomyces ...

Automated determination of fatty acid methyl ester and cis/trans methyl ester composition of fats and oils.

The determination of the fatty acid composition (as methyl esters, FAMEs) of fats and oils and their cis/trans (CTME) distribution requires a simple, but manual and time-consuming sample preparation. The so-called BF3 method is often the preferred procedure. Because FAME/CTME analyses are encountered very frequently in the food industry, an automated, robot-based alternative is proposed which u...

Fatty Acid Composition in Microalgae'

The degree of unsaturation of fatty acids was higher in ChIorella vulgaris 1 h cells grown with air (low-CO2 cells) than in the cells grown with air enriched with 2% CO2 (high-CO2 cells). The change in the ratio of linoleic acid to a-linolenic acid was particularly significant. This change of the ratio was observed in four major lipids (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, ...