Hemagglutination Inhibition Test for Detection of Infectious Bovine Rhinotracheitis Virus Infection in the Field

Susceptibility of bovine macrophages to infectious bovine rhinotracheitis virus infection.

Infectious bovine rhinotracheitis virus replicated in cultured bovine alveolar macrophages (AM). However, yields of infectious virus were low, with maximum titers approximately 100 times that of the residual inoculum. Immunofluorescence and electron microscopic studies indicated that the majority of macrophages produced viral antigen, but after infection at a multiplicity of 0.1, only 4.1% of A...

Detection of Infectious Bronchitis Virus in Allantioc Fluid by Rapid Hemagglutination Test.

To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...

The Isolation and Identification of Infectious Bovine Rhinotracheitis Virus in Iran

Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis.

Bovine herpesvirus 1 (BoHV-1), classified as an alphaherpesvirus, is a major pathogen of cattle. Primary infection is accompanied by various clinical manifestations such as infectious bovine rhinotracheitis, abortion, infectious pustular vulvovaginitis, and systemic infection in neonates. When animals survive, a life-long latent infection is established in nervous sensory ganglia. Several react...

Infectious bovine rhinotracheitis.

Cattle exhibiting the vulvovaginitis form of the IBR complex are sexually mature females that do not appear ill. Signs of IPV include a thick yellow to brown vulvar discharge that attaches to the vulvar tuft of hair. The vulva is swollen and the vulvar and vaginal lining is reddened, dying and/or contains small whitish-colored pustules. The vaginal-vulvar infection causes irritation, exhibited ...