High CD33 expression levels in acute myeloid leukemia cells carrying the nucleophosmin (NPM1) mutation

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High CD33 expression levels in acute myeloid leukemia cells carrying the nucleophosmin (NPM1) mutation.

The CD33 antigen is expressed on the blast cells of most cases of acute myeloid leukemia and represents a suitable tumor-associated target antigen for antibody-based therapies. The aim of this study was to investigate the relationship between the CD33 levels quantified by mean fluorescence intensity and antibody binding capacity, and the presence/absence of NPM1 and FLT3 gene mutations in 99 ne...

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Nucleophosmin gene mutation in acute myeloid leukemia patients.

A myeloid leukemia (AML) is a heterogeneous group of clonal hematopoietic stem cell disorders. Numerous recurrent structural and numeric cytogenetic aberrations have been identified, and many of them are not only diagnostic markers for specific AML subtypes, but also constitute independent prognostic factors for attainment of complete remission (CR), relapse risk, and overall survival (OS). Mor...

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The Difference in Initial Leukocyte Count, Bone Marrow Blast Cell Count and CD 34 Expression in Patients with Acute Myeloid Leukemia with and without NPM1 gene Mutation

Background: Mutation in NPM1 gene has been reported to be the most common genetic mutation in de novo acute myeloid leukemia (AML). AML with NPM1 gene mutation usually presents with higher initial leukocyte and blast cell counts and negative CD34 expression. We aimed to investigate the difference of initial leukocyte counts, bone marrow blast cell counts and expression of CD34 among patients wi...

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Circular RNAs of the nucleophosmin (NPM1) gene in acute myeloid leukemia

In acute myeloid leukemia, there is growing evidence for splicing pattern deregulation, including differential expression of linear splice isoforms of the commonly mutated gene nucleophosmin (NPM1). In this study, we detect circular RNAs of NPM1 and quantify circRNA hsa_circ_0075001 in a cohort of NPM1 wild-type and mutated acute myeloid leukemia (n=46). Hsa_circ_0075001 expression correlates p...

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Cytoplasmic expression of nucleophosmin accurately predicts mutation in the nucleophosmin gene in patients with acute myeloid leukemia and normal karyotype.

Mutations in the nucleophosmin (NPM1) exon 12 resulting in delocalization of NPM1 into the cytoplasm occur in 50% to 60% of acute myeloid leukemia cases with a normal karyotype (AML-NK). As recent studies suggest such patients have a favorable prognosis and there are discordant reports of the immunohistochemical detection of cytoplasmic NPM1 (NPMc+) for predicting NPM1 gene mutations, we correl...

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ژورنال

عنوان ژورنال: Haematologica

سال: 2011

ISSN: 0390-6078,1592-8721

DOI: 10.3324/haematol.2011.043786