High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980
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چکیده
منابع مشابه
Stable low-copy-number Staphylococcus aureus shuttle vectors.
A series of Staphylococcus aureus-Escherichia coli shuttle vectors were constructed which contained the replication and maintenance functions of the S. aureus theta-mode multiresistance plasmid pSK1. The utility of the newly constructed vectors was demonstrated by the successful cloning and expression of several genes that had previously proven difficult to express in S. aureus. Additional vect...
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A number of genetic and biotechnological applications are facilitated by the ability to transform Escherichia coli with more than one recombinant plasmid. For example, a plasmid that carries a gene of interest may require that a trans-acting regulatory protein be expressed from a second plasmid in the cell to effectively regulate its expression (9,16,29). In addition, purification of multisubun...
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Small plasmids which replicate in both Escherichia coli and Clostridium perfringens were made by recombining E. coli plasmid pBR322 with three different small (less than 4 kilobases) plasmids native to C. perfringens. Subsequently, two homologous, though distinct, tetracycline resistance determinants (tet) from other C. perfringens plasmids were cloned into them. Both tet systems made E. coli r...
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Low copy number plasmids cannot rely on the random segregation during bacterial cell division. To be stably maintained in the population they evolved two types of mechanisms (i) partition systems (PAR) that actively separate replicated plasmid molecules to the daughter cells and (ii) toxin-andidote systems (TA) that act after cell division to kill plasmid-less cells. Our knowledge of partition ...
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2.Bailey, J.E., N.A. Da Silva, S.W. Peretti, J.H. Seo and F. Srienc. 1986. Studies of hostplasmid interactions in recombinant microorganisms. Ann. NY Acad. Sci. 469:194-211. 3.Lerner, C.G. and C.D. Carter. 1995. PCR colony screening using the scintillation proximity assay to detect inserts in cloning vectors. BioTechniques 19:914-917. 4.Liu, Z. and N.C. Mishra. 1995. Single-tube method for plas...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2020
ISSN: 1475-2859
DOI: 10.1186/s12934-020-1296-5