High lumenal chloride in the lysosome is critical for lysosome function
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چکیده
منابع مشابه
High lumenal chloride in the lysosome is critical for lysosome function
Lysosomes are organelles responsible for the breakdown and recycling of cellular machinery. Dysfunctional lysosomes give rise to lysosomal storage disorders as well as common neurodegenerative diseases. Here, we use a DNA-based, fluorescent chloride reporter to measure lysosomal chloride in Caenorhabditis elegans as well as murine and human cell culture models of lysosomal diseases. We find tha...
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Recent data both from cell-free experiments and from cultured cells have shown that lysosomes can fuse directly with late endosomes to form a hybrid organelle. This has a led to a hypothesis that dense core lysosomes are in essence storage granules for acid hydrolases and that, when the former fuse with late endosomes, a hybrid organelle for digestion of endocytosed macromolecules is created. L...
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Autophagosome-lysosome fision measured by means o f electroinjected [ ‘“C] lactose While [‘“C] sucrose is an inert sequestration probe which eventually accumulates in lysosomes, [‘“c] lactose is hydrolysed immediately upon entry into the lysosome, by the lysosomal enzyme 0-galactosidase. Autophagically sequestered [“C] lactose therefore rapidly equilibrates at a low steady-state level, probably...
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ژورنال
عنوان ژورنال: eLife
سال: 2017
ISSN: 2050-084X
DOI: 10.7554/elife.28862