High Performance Liquid Chromatography and Its Validation - Review

Vortex-Assisted Inverted Dispersive Liquid-Liquid Microextraction of Naproxen from Human Plasma and Its Determination by High-Performance Liquid Chromatography

Extraction and determination of naproxen from human plasma were performed using Vortex-Assisted Inverted Dispersive Liquid-Liquid MicroExtraction (VA−IDLLME) and High-Performance Liquid Chromatography (HPLC). The parameters affecting extraction recovery such as type and volume of extraction and disperser solvents, pH of sample solution, stirring rate, extraction time and salt addition were ...

Denaturing high-performance liquid chromatography: A review.

Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial denaturation. Temperature determines sensitivity, and its optimum can be predicted by computation...

Method Development and Validation of Carisoprodol and its Impurities by Ultra Violet-High Performance Liquid Chromatography

Ultraviolet-h igh performance liquid chromatographic (UV-HPLC) method was developed for the quantitation of carisoprodol and its impurities viz 2-methyl-2-propylpropane-1, 3-diyl d icarbamate (impurity-D) and N-isopropyl -2-methyl-2-propyl-3-hydroxy propyl carbamate (impurity-B) in active pharmaceutical ingredient. Validation of the method showed excellent sensitivity, selectivity, accuracy, pr...

development and validation of high performance liquid chromatography for determination of nystatin in pharmaceutical formulations

High-Performance Liquid Chromatography Determination of Methotrexate in Plasma

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...