High-throughput mutation detection underlying adaptive evolution of Escherichia coli-K12
نویسندگان
چکیده
منابع مشابه
Directed evolution of cellobiose utilization in Escherichia coli K12.
The cellobiose catabolic system of Escherichia coli K12 is being used to study the role of cryptic genes in evolution of new functions. Escherichia coli does not use beta-glucoside sugars; however, mutations in several loci can activate the cryptic bgl operon and permit growth on the beta-glucoside sugars arbutin and salicin. Such Bgl+ mutants do not use cellobiose, which is the most common bet...
متن کاملAdaptive mutation in Escherichia coli.
In 1988 John Cairns, Julie Overbaugh, and Stephan Miller published a paper entitled “The origin of mutants,” in which they suggested that bacteria could choose which mutations to make (11). Shortly thereafter, Cairns and I began collaborating on a National Science Foundation-funded project to investigate the genetic basis of what was popularly called “directed mutation” (although at the time we...
متن کاملRebuttal: adaptive mutation in Escherichia coli (Foster).
If mutations arose in nongrowing cells, as proposed in Foster’s paper (1), then stress-induced mutagenesis would seem inescapable. The plated population certainly grows very little. However, no evidence is provided that reversion occurs in this population and no effort is made to counter evidence to the contrary (see below)—that mutations actually arise in cells growing under selection within d...
متن کاملAdaptive evolution of genomically recoded Escherichia coli.
Efforts are underway to construct several recoded genomes anticipated to exhibit multivirus resistance, enhanced nonstandard amino acid (nsAA) incorporation, and capability for synthetic biocontainment. Although our laboratory pioneered the first genomically recoded organism (Escherichia coli strain C321.∆A), its fitness is far lower than that of its nonrecoded ancestor, particularly in defined...
متن کاملAdaptive reversion of a frameshift mutation in Escherichia coli.
Mutation rates are generally thought not to be influenced by selective forces. This doctrine rests on the results of certain classical studies of the mutations that make bacteria resistant to phages and antibiotics. We have studied a strain of Escherichia coli which constitutively expresses a lacI-lacZ fusion containing a frameshift mutation that renders it Lac-. Reversion to Lac+ is a rare eve...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2004
ISSN: 1088-9051
DOI: 10.1101/gr.2977704