High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

BACKGROUND Some strains of Bacillus licheniformis have been improved by target-directed screening as well as by classical genetic manipulation and used in commercial thermostable alpha-amylase and alkaline protease production for over 40 years. Further improvements in production of these enzymes are desirable. RESULTS A new strain of B. licheniformis CBBD302 carrying a recombinant plasmid pHY...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Two-step purification and partial characterization of an extra cellular α-amylase from Bacillus licheniformis

The aim of this study was production and partial purification of α-amylase enzyme by Bacillus licheniformis. B. Licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. Optimal conditions for amylase production by B. Licheniformis are incubation period of 120 h, temperature of 37 °C and pH 7.0. The α-amylase enzyme was purified by ion exchange chromatography ...

Improved thermostable α-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation.

Thermostable α-amylase is of great importance in the starch fermentation industry; it is extensively used in the manufacture of beverages, baby foods, medicines, and pharmaceuticals. Bacillus amyloliquefaciens produces thermostable α-amylase; however, production of thermostable α-amylase is limited. Ion-beam implantation is an effective method for mutation breeding in microbes. We conduct...

Thermophilic Bacillus Licheniformis Rbs 5 Isolated from Hot Tunisian Spring Co-producing Alkaline and Thermostable Α-amylase and Protease Enzymes