HLA-B27 screening by flow cytometry
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چکیده
منابع مشابه
HLA-B27 screening by flow cytometry.
A flow cytometric assay for lymphocyte HLA-B27 expression using a two-color direct immunofluorescent assay was compared to traditional microlymphocytotoxicity testing on 209 clinical samples. For the flow cytometric assay, whole blood was mixed with a monoclonal anti-B27 conjugated to fluorescein-isothiocyanate (FITC) and anti-CD3 conjugated to phycoerythrin (PE). The samples were analyzed with...
متن کاملHLA-B27 phenotyping with flow cytometry: further improvement by multiple monoclonal antibodies.
To establish the optimal flow cytometric method for HLA-B27 phenotyping, we compared several strategies, using three monoclonal anti-B27 antibodies (from the HLA-ABC-m3, GS145.2, and FD705 clones). We used a triple-color direct immunofluorescence assay, including a T-lymphocyte-specific antibody as an internal control and an anti-HLA-Bw4 antibody. Blood samples from >400 subjects were tested. F...
متن کاملSulphydryl reactivity of the HLA-B27 epitope: accessibility of the free cysteine studied by flow cytometry.
HLA-B27 has an unpaired cysteine on or near its serologically defined spondylitis associated epitope, and it has been argued that its sulphydryl side chain may be chemically reactive. In a previous study it was shown that chemical treatment of HLA-B27 cells with the sulphydryl binding agent p-chloromercuriphenylsulphonic acid (pCMPSA) specifically reduced binding of antibodies to HLA-B27 by up ...
متن کاملHLA-B27 detection – comparison of genetic sequence-based method and flow cytometry assay
OBJECTIVES The presence of human leukocyte antigen B27 (HLA-B27) is strongly associated with ankylosing spondylitis. HLA-B27 testing is routinely applied in the diagnosis of this disease. The aim of the present study was to compare two methods of HLA-B27 detection - a genetic sequence-based method and a flow cytometry assay. MATERIAL AND METHODS Peripheral blood was obtained from 300 individu...
متن کاملHLA-B27 typing by use of flow cytofluorometry.
We describe the use of flow cytofluorometry to type lymphocytes for HLA-B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts wit...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1995
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990220211