Hyphenated NMR Methods in Natural Products Research, Part 2: HPLC-SPE-NMR and Other New Trends in NMR Hyphenation
نویسندگان
چکیده
منابع مشابه
HPLC-SPE-NMR: a productivity tool in natural products research
Natural products provide excellent potential leads for drug development because of their chemical diversity and biological functionality. However, the productivity of discovery of new, pharmacologically active natural products has traditionally been low due to inherent difficulties and costs associated with extract dereplication, i.e., isolation, purification and structure elucidation of indivi...
متن کاملHPLC-SPE-NMR: a productivity tool in natural products research
Natural products provide excellent potential leads for drug development because of their chemical diversity and biological functionality. However, the productivity of discovery of new, pharmacologically active natural products has traditionally been low due to inherent difficulties and costs associated with extract dereplication, i.e., isolation, purification and structure elucidation of indivi...
متن کاملHPLC-SPE-NMR hyphenation in natural products research: optimization of analysis of Croton membranaceus extract.
The HPLC-SPE-NMR technique was used for the analysis of a root-bark extract of Croton membranaceus. The components of the extract were separated on an analytical-size reversed-phase HPLC column, the chromatographic peaks were trapped on SPE (solid-phase extraction) cartridges after post-column dilution of the eluate with water and the compounds were eluted from the cartridges with acetonitrile-...
متن کاملImportant roles of the hyphenated HPLC-DAD-MS-SPE-NMR technique in metabonomics.
Metabolite identification is a key step for metabonomics study. A fully automated hyphenation of HPLC-diode-array detector (DAD) mass spectrometry (MS) solid phase extraction (SPE)-NMR spectroscopy (HPLC-DAD-MS-SPE-NMR) is one of the most efficient methods to determine the structure of a given unknown metabolite in a complex mixture (metabonome) and hence represents one of the most important an...
متن کاملHPLC-SPE-NMR in pharmaceutical development: capabilities and applications.
High-performance liquid chromatography-solid phase extraction-NMR spectroscopy (HPLC-SPE-NMR) has recently become commercially available and has been evaluated with regard to its applicability in a pharmaceutical environment. The addition of an automated SPE unit to an HPLC-NMR system for peak trapping results in an improved NMR signal-to-noise ratio (S/N) and also has other practical advantage...
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ژورنال
عنوان ژورنال: Planta Medica
سال: 2005
ISSN: 0032-0943,1439-0221
DOI: 10.1055/s-2005-873114