Identification and in vitro Characterization of a Novel Phage Endolysin that Targets Gram-Negative Bacteria
نویسندگان
چکیده
منابع مشابه
In vitro characterization of PlyE146, a novel phage lysin that targets Gram-negative bacteria
The recent rise of multidrug-resistant Gram-negative bacteria represents a serious threat to public health and makes the search for novel effective alternatives to antibiotics a compelling need. Bacteriophage (Phage) lysins are enzymes that hydrolyze the cell wall of bacteria and represent a promising alternative to tackle this ever-increasing problem. Despite their use is believed to be restri...
متن کاملSynthesis and Characterization of Novel Antibacterial PDDA/Honey Nanofiber Against Gram-Positive and Gram-Negative Bacteria
Nanomaterials are increasingly used to the targeting of gram-positive and gram-negative bacteria as an alternative to antibiotics. Bacterial infections are a major cause of chronic infections and mortality. People requirement for new materials for pathogenic bacteria treatment. It seems that nanomaterial-based strategies can be resolving this problem. In this research, improved antibacterial na...
متن کاملFunctional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram-negative bacteria.
To analyze the antibacterial activity of Bacillus amyloliquefaciens phage endolysin, nine deletion derivatives of the endolysin were constructed. Each deletion mutant was overexpressed, purified and characterized. The catalytic domain was located on the N-terminal region and the C-terminus had an affinity with the bacterial envelope. The enzymatic activity remained in spite of the deletion of t...
متن کاملStructural and Enzymatic Characterization of ABgp46, a Novel Phage Endolysin with Broad Anti-Gram-Negative Bacterial Activity
The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly against multidrug resistant strains of Acinetobacter baumannii. We have cloned, heterologously expressed and characterized a novel endolysin (ABgp46) from Acinetobacter phage vb_AbaP_CEB1 and tested its antibacterial activity against several multidrug-resistant A. bauman...
متن کاملCharacterization and identification of gram-negative, nonfermentative bacteria.
The morphological and physiological characteristics of 593 strains of nonfermentative, gram-negative bacteria are described. A battery of 46 tests was used to identify and differentiate strains representing 8 genera and 31 species of named and group-designated bacteria. Seven selected amides and organic salts were closely examined to determine their usefulness, individually or as a battery, in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microorganisms
سال: 2020
ISSN: 2076-2607
DOI: 10.3390/microorganisms8030447