Identification and molecular cloning of a gene encoding a fibronectin‐binding protein (CadF) from Campylobacter jejuni
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چکیده
منابع مشابه
Cloning and molecular characterization of TaERF6, a gene encoding a bread wheat ethylene response factor
Ethylene response factor proteins are important for regulating gene expression under different stresses. Different isoforms for ERF have previously isolated from bread wheat (Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5. We isolated, cloned and molecular characterized a novel one based on TdERF1, an isoform in durum wheat (Tri...
متن کاملA Molecular Survey of Campylobacter jejuni and Campylobacter Coli Virulence and Diversity
Background: The aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis of Campylobacter spp. isolated from children with diarrhea in Iran. Methods: A total of 200 stool specimens were obtained from children under 5 years during July 2012 to July 2013. Detection of C. jejuni and C. coli was perfo...
متن کاملCloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from Campylobacter jejuni and Campylobacter coli.
Genes encoding superoxide dismutase (SOD: EC 1.15.1.1) were cloned from Campylobacter jejuni NCTC 11351 and Campylobacter coli UA585 by heterologous complementation of a SOD-deficient Escherichia coli mutant. Deletion analysis of the cloned C. jejuni DNA assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. The deduced gene product of 220 amino acids was 7...
متن کاملIdentification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product.
Campylobacter jejuni and Campylobacter coli are common causes of gastroenteritis in humans. Infection with C. jejuni or C. coli is commonly acquired by eating undercooked chicken. The goal of this study was to develop specific detection assays for C. jejuni and C. coli isolates based on the cadF virulence gene and its product. The cadF gene from C. jejuni and C. coli encodes a 37-kDa outer memb...
متن کاملin silico analysis of the cadf gene and development of a duplex polymerase chain reaction for species-specific identification of campylobacter jejuni and campylobacter coli
materials and methods in silico analysis of the cadf gene was carried out by several software and available online tools. a duplex pcr optimized with specific primers was used for detection and differentiation of both species. to evaluate specificity and sensitivity of the test, a panel of different campylobacter spp. together with several intestinal bacterial pathogens was tested. the limit of...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1997
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.1997.4031771.x