Identification of Glycosyltransferases Focusing on Golgi Transmembrane Region
نویسندگان
چکیده
منابع مشابه
Distribution of terminal glycosyltransferases in hepatic Golgi fractions
The distribution of the three glycosyltransferases synthesizing the terminal trisaccharide sialic acid yields D-galactose yields N-acetylglucosamine present in many glycoproteins was determined in Golgi fractions prepared from rat liver homogenates by a modification of the procedure of Ehrenreich et al. (1973, J. Cell Biol. 70:671--684). The enzymes were assayed with asialofetuin, ovomucoid, an...
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Glycosylation is one of the major post-translational modification processes essential for expression and function of many proteins. It has been estimated that 1% of the open reading frames of a genome is dedicated to glycosylation. Many different enzymes are involved in glycosylation, such as glycosyltransferases and glycosidases. Traditionally, glycosyltransferases are classified based on thei...
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Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta 1,4 galactosyltransferase (GalT) and the medial enzyme, N-acetylglucosaminyltransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans-G...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 2007
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.19.41