Identification of Medically Important Candida Species by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of the rDNA ITS1 and ITS2 Regions
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چکیده
منابع مشابه
Identification of screwworm species by polymerase chain reaction-restriction fragment length polymorphism.
Restriction fragment length polymorphisms in polymerase chain reaction amplified fragments (PCR-RFLP) of mitochondrial DNA were used to differentiate species of New World screwworms (Diptera: Calliphoridae). Twenty-seven restriction enzymes were screened on five regions of mtDNA. Eleven restriction fragment length patterns differentiated New World screwworm, Cochliomyia hominivorax (Coquerel), ...
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Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal speci...
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BACKGROUND Transgenic research on metalloproteinase-1 is an emerging field in the area of plant molecular biology. The new method reported here can similarly be applied in fungal molecular biology to identify different dermatophytes. Our method is more accurate than traditional methods such as molecular analyses. OBJECTIVE To identify Trichophyton rubrum, T. mentagrophytes var. mentagrophytes...
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Prompt and specific identification of fungemia agents is important in order to define clinical treatment. However, in most cases conventional culture identification can be considered to be time-consuming and not without errors. The aim of the present study was to identify the following fungemia agents: Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Cryptococcus ne...
متن کاملDiagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids
BACKGROUND/AIMS The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid and inexpensive technique for identifying Mycobacterium spp. METHODS We performed PCR-RFLP to detect and identify Mycobacterium spp....
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2017
ISSN: 0255-0857
DOI: 10.4103/ijmm.ijmm_17_102