Identification of New Antimicrobial Peptides that Contribute to the Bactericidal Activity of Egg White against <i>Salmonella enterica</i> Serovar Enteritidis at 45 °C

A recent work revealed that egg white (EW) at 45 °C exhibits powerful bactericidal activity against S. enterica serovar Enteritidis, which is surprisingly little affected by removal of the >10 kDa EW proteins. Here, we sought to identify major factors responsible for this fractionating using ultrafiltration and nanofiltration characterizing physicochemical antimicrobial properties resulting fractions. In particular, 22 peptides were identified nano-LC/MS-MS activities representative (with predicted activity) further assessed. Two (FVPPVQR GDPSAWSWGAEAHS) found be Enteritidis when provided in an environment. Nevertheless, these contribute only part activity, suggesting other, yet determined, factors.