Identification of phosphorylation sites in rat liver CTP: phosphocholine cytidylyltransferase.
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چکیده
منابع مشابه
Identification of phosphorylation sites in rat liver CTP: phosphocholine cytidylyltransferase.
CTP:phosphocholine cytidylyltransferase (CT) is an important regulatory enzyme in phosphatidylcholine biosynthesis. The enzyme exists as a soluble, inactive form that is highly phosphorylated; activation of the enzyme is accompanied by dephosphorylation and translocation to the membrane. We have used a recombinant baculovirus clone to obtain CT labeled in vivo with 32PO4. The tryptic phosphopep...
متن کاملCloning and expression of rat liver CTP: phosphocholine cytidylyltransferase: an amphipathic protein that controls phosphatidylcholine synthesis.
CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) is a key regulatory enzyme in the synthesis of phosphatidylcholine in higher eukaryotes. This enzyme can interconvert between an inactive cytosolic form and an active membrane-bound form. To unravel the structure of the transferase and the mechanism of its interaction with membranes, we have cloned a cytidylyltransferase cDNA from rat liver ...
متن کاملRegulation of CTP: phosphocholine cytidylyltransferase activity in type II pneumonocytes.
Phosphatidylcholine synthesis by rat type II pneumonocytes was altered either by depleting the cells of choline or by exposing the cells to extracellular lung surfactant. Effects of these experimental treatments on the activity of a regulatory enzyme, CTP:phosphocholine cytidylyltransferase, were investigated. Although choline depletion of type II pneumonocytes resulted in inhibition of phospha...
متن کاملCTP: Phosphocholine cytidylyltransferase: paving the way from gene to membrane.
PC is the most abundant phospholipid in eukaryotic membranes whereas few prokaryotes contain PC. PC is synthesized in eukaryotes by two routes: the CDP-choline pathway (1) and PE methylation (2). PE methylation is the most important contributor to PC formation in Saccharomyces cerevisiae (3). In contrast, mammalian cells and tissues predominantly use the CDP-choline pathway (2, 4). PC is the ma...
متن کاملActivation of CTP : phosphocholine cytidylyltransferase in rat lung by fatty acids.
CTP : phosphocholine cytidylyltransferase activity exists in both the microsome and cytosol fractions of adult lung, 36 and 59%, respectively. Although these enzyme activities are stimulated in vitro by added lipid activators (i.e. phosphatidylglycerol), there are significant levels of activity in the absence of added lipid. We have removed endogenous lipid material from microsome and cytosol p...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34092-9