Identification of whitefly (Bemisia tabaci) proteins interacting with Tomato leaf curl Bangalore virus coat protein gene using Y2H system

Tomato leaf curl Bangalore virus (ToLCBV) (Geminiviridae) causes the economically important tomato disease (ToLCVD), and is transmitted by whitefly, Bemisia tabaci (Gennadius) (Aleyrodidae: Hemiptera). Successful transmission of insect requires safe translocation through different barriers inside vector. During translocation, viral coat protein will interact with many whitefly proteins, while a few them are beneficial to (e.g. heat shock proteins) but some may be harmful immune proteins). We carried out yeast two hybrid (Y2H) assays identify proteins B.tabaci Asia 1 genetic group interacting ToLCBV (CP). The Y2H assay initially identified total 425 putative on low stringent selection media, they were reduced 324 when colonies grown high which about 274 produced single bands in colony PCR experiments remaining multiple bands. Further, pressure confirmed 102 CP these included (HSPs) 70 kDa, GroEL, nucleoproteins, vitellogenins, apolipophorins, 40 s ribosomal sorbitol dehydrogenase, dipeptidyl peptidase, E3 ubiquitin, annexin, GTP cyclohydrolase, tropomyosin, salivary secreted succinate lachesins, enolase others. could potential targets for novel control strategies such as using RNAi or insecticide target sites developing future strategies.