Imaging flow cytometry analysis of intracellular pathogens

Flow Cytometry Analysis of Intracellular Protein

The demand for determining protein levels in a continuously decreasing amount of input cells is currently being developed by scientists and medical doctors. Another demanding area is the splitting of active and inactive (phosphorylated and unphosphorylated, respectively) forms of the protein being monitored, e.g., during different phases of the cell cycle, differentiation or carcinogenesis. The...

Cell Interaction Analysis by Imaging Flow Cytometry

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P-95: Flow Cytometry Analysis of Bovine Semen:A Qualitative Study

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...

Intracellular antigen determination by flow cytometry in leukaemic cells.

Several fixation and permeabilization techniques that enable the flow cytometric analysis of the cell contents have been introduced in recent years. These methods allow sensitive detection of intracellular antigens that facilitates the diagnosis of certain diseases. We have undertaken in this study to evaluate a simple method of fixation and permeabilization using 2% paraformaldehyde and Tween ...

Intracellular Flow Cytometry - Multiparameter analysis of cytokine, transcription factor, and phosphoprotein expression