Immobilized Enzymes on Graphene as Nanobiocatalyst
نویسندگان
چکیده
منابع مشابه
Graphene oxide immobilized enzymes show high thermal and solvent stability.
The thermal and solvent tolerance of enzymes is highly important for their industrial use. We show here that the enzyme lipase from Rhizopus oryzae exhibits exceptionally high thermal stability and high solvent tolerance and even increased activity in acetone when immobilized onto a graphene oxide (GO) nanosupport prepared by Staudenmaier and Brodie methods. We studied various forms of immobili...
متن کاملCoupled reactions of immobilized enzymes and immobilized substrates: clinical application as exemplified by amylase assay.
We described a partitioned enzyme-sensor system, which incorporates an immoblized substrate and three or more discrete immobilized enzymes. This instrument measures alpha-amylase activity by passing the solution containing alpha-amylase over a column packed with immobilized starch. The resulting oligosaccharides are successively exposed to a column or columns containing immobolized glucose oxid...
متن کاملImmobilized enzymes: crystals or carriers?
The advantages of immobilized over soluble enzymes arise from their enhanced stability and ease of separation from the reaction media, leading to significant savings in enzyme consumption. Immobilization methods range from binding to prefabricated carrier materials to packaging in enzyme crystals or powders. During their use, mass-transfer effects can produce substrate or pH gradients, which re...
متن کاملAnalytical applications of immobilized enzymes.
Several clinical laboratory methods using enzymes as reagents now may utilize immobilized enzymes. These are enzymes attached to solid surfaces by adsorption, covalent binding, cross linking or similar means. Immobilized enzymes are widely used presently in convenient tests for urine glucose and galactose or blood glucose and urea, and in serum glucose or urea determinations by automated method...
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ژورنال
عنوان ژورنال: ACS Applied Materials & Interfaces
سال: 2019
ISSN: 1944-8244,1944-8252
DOI: 10.1021/acsami.9b17777