IMMU-21. GLIOMA-DERIVED FACTORS RECRUIT AND INDUCE AN IMMUNE SUPPRESSIVE PHENOTYPE IN BONE MARROW-DERIVED CCR2+ MYELOID CELLS
نویسندگان
چکیده
Abstract INTRODUCTION Infiltrating immune-suppressive myeloid cells represent a tumor supportive population that contributes to immune checkpoint inhibitor resistance and poor survival in Glioblastoma (GBM) patients. We have previously characterized monocytic-myeloid derived suppressor (M-MDSCs) based on their dual expression of chemokine receptors CCR2 CX3CR1(CCR2+/CX3CR1+). Genetic pharmacologic targeting CCR2, combination with PD-1 blockade, reduced the percentage M-MDSCs glioma-microenvironment slowed progression KR158 005GSC murine gliomas. Additional studies are needed investigate chemokines responsible for recruitment CCR2+/CX3CR1+ impact glioma factors suppressive phenotype. OBJECTIVE Evaluate effect migration suppression bone marrow cells. METHODS A transwell assay was utilized determine migratory ability KR158B conditioned presence CCL2 CCL7 neutralizing antibodies. Ly6G-/GR1+ were isolated from cultured media co-cultured freshly T-cells examine RESULTS gliomas differentially upregulate cytokines including CCL2, IL6, G-CSF, GM-CSF as compared healthy naive brains. increased marrow-derived CD11b+, Ly6Chi, Ly6G-. Bone expanded condition suppress both CD4+ CD8+ T cell proliferation. migrate recombinant well media. Migration is completely inhibited by High associated prognosis human GBM. CONCLUSION Glioma-derived mediate CCR2+ into microenvironment redundant manner. glioma-derived induce CD4/8+ state.
منابع مشابه
Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells
Objective(s): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. Materials and Methods: ...
متن کاملImmune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells
Mesenchymal stem cells (MSCs) are considered valuable sources for cell therapy because of their immune regulatory function. Here, we investigated the effects of tonsil-derived MSCs (T-MSCs) on the differentiation, maturation, and function of dendritic cells (DCs). We examined the effect of T-MSCs on differentiation and maturation of bone-marrow- (BM-) derived monocytes into DCs and we found sup...
متن کاملHemozoin Enhances Maturation of Murine Bone Marrow Derived Macrophages and Myeloid Dendritic Cells
Background: Falciparum malaria is a severe health burden worldwide. Antigen presenting cells are reported to be affected by erythrocytic stage of the parasite. Malarial hemozoin (HZ), a metabolite of malaria parasite, has adjuvant properties and may play a role in the induction of immune response against the parasite. Objective: To determine the immunological impact of hemozoin on the capacity ...
متن کاملDifferentiation of Adipose-derived Stem Cells into Schwann Cell Phenotype in Comparison with Bone Marrow Stem Cells
Objective(s) Bone marrow is the traditional source of human multipotent mesenchymal stem cells (MSCs), but adipose tissue appears to be an alternative and more readily available source. In this study, rat adipose-derived stem cells (ADSCs) were induced to differentiate into Schwann-like cells and compared with rat bone marrow stem cells (BMSCs) for their Schwann-like cells differentiation pote...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Neuro-oncology
سال: 2022
ISSN: ['1523-5866', '1522-8517']
DOI: https://doi.org/10.1093/neuonc/noac209.519