Immuno-affinity purification of 2B8-tagged proteins
نویسندگان
چکیده
منابع مشابه
High-throughput Purification of Affinity-tagged Recombinant Proteins
X-ray crystallography is the method of choice for obtaining a detailed view of the structure of proteins. Such studies need to be complemented by further biochemical analyses to obtain detailed insights into structure/function relationships. Advances in oligonucleotide- and gene synthesis technology make large-scale mutagenesis strategies increasingly feasible, including the substitution of tar...
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A magnetic sorbent with stable and superior magnetic behaviour was developed for His-tagged protein purification by immobilized metal affinity chromatography (IMAC). Magnetic, monodisperse and porous silica microspheres 6 mm in size, with bimodal pore size distribution including both mesoporous and macroporous compartments were synthesized as the base material by a staged-shape template hydroly...
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ژورنال
عنوان ژورنال: Applied Biological Chemistry
سال: 2017
ISSN: 2468-0834,2468-0842
DOI: 10.1007/s13765-017-0310-z