Immuno-Dot Blot Analysis of a Monoclonal Antibody that Blocks the Human Monocyte Response to Migration Inhibitory Factor

An inhibitory monoclonal antibody to factor X that blocks prothrombin activation but not prothrombinase enzyme assembly.

A monoclonal antibody (designated alpha BFX-2b) prepared against bovine factor X inhibited factor X activity in human, bovine, porcine, rabbit, and canine plasma. In assays using purified prothrombinase components, factor Xa, factor Va, phospholipid vesicles, and calcium ion with the fluorescent active site thrombin inhibitor dansylarginyl-N-(3-ethyl-1,5-pentanediyl)amide, the antibody inhibite...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

Development of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus

Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...

The production of a monoclonal antibody that blocks the action of a neurite outgrowth-promoting factor.

How growing axons are guided to their targets has been the subject of much speculation. A popular hypothesis is that there are macromolecules in the extracellular matrix (ECM) or on the surfaces of other cells that form pathways for axon guidance. Such molecular signals could act via a number of different mechanisms. One way of directing neurite growth is by providing a very adhesive surface fo...

An inhibitory monoclonal antibody against human tissue factor.

We obtained a hybridoma using immune spleen cells from a mouse injected with human brain tissue factor that had been purified on a factor VII-agarose affinity column. This monoclonal IgG1, HTF1-7B8, inhibits tissue factor procoagulant activity. The concentration of HTF1-7B8 producing half-maximal inhibition is influenced by the concentration of factor VIIa, suggesting that the antibody and enzy...