Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR

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Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR.

To assess the risks from viral contamination of drinking-water supplies, there is a clear need for methods to directly detect viral pathogens. In this study, we developed a broad-spectrum immunocapture method for concentration and purification of enteric viruses. The method involved indirect antibody capture (AbCap) of intact viruses followed by release of virion genomic RNA and reverse transcr...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1996

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.62.6.2086-2094.1996