Immunochemical extraction and automated measurement of plasma creatine kinase MB isoenzyme and creatine kinase MB2 isoform
نویسندگان
چکیده
منابع مشابه
Creatine and creatine kinase measurement.
*Many methods for measuring creatine are known, the principal ones being direct calorimetric assay (l), indirect calorimetric assay (2), and destruction with bacterial adaptive enzymes (3). However, none of these methods are specific nor are they sensitive enough to detect very small amounts of creatine. rIhese assays have been used to examine urine and serum for creatine content, but, because ...
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Serial measurements of the MB isoenzyme of creatine kinase, total creatine kinase, aspartate mainotransferase, and urea stable lactate dehydrogenase have been made in the serum of a series of 139 patients admitted with a diagnosis of suspected myocardial infarction. Serum MB isoenzyme measurements have also been made on postoperative surgical patients and patients with medical disorders known t...
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Several methods are available for specifically identifying and assessing the activity of MB isoenzyme of creatine kinase in serum. We compared two electrophoretic techniques with three column-chromatographic ones for efficacy in the diagnosis of myocardial infarction. No technique was considered absolute, but we found surprisingly large errors of diagnosis in the column-chromatographic techniqu...
متن کاملAn immunochemical mass assay for the direct measurement of creatine kinase MB2.
The isoforms of CK-MB have recently received attention as potential biochemical markers for the early diagnosis of an acute myocardial infarction. A sensitive (analytical sensitivity = 0.2 ng/ml) immunochemical mass assay for the direct measurement of the CK-MB2 isoform has been developed by us. This assay utilizes a specific monoclonal capture antibody directed against the B-subunit of CK-MB a...
متن کاملHigh creatine kinase MB isoenzyme activity associated with a rhabdomyosarcoma.
A 20-year-old man was found to have high plasma creatine kinase (EC 2.7.3.2) activity in association with a rhabdomyosarcoma of the prostate. There was a very high proportion of the MB isoenzyme. Clinical findings and changes in creatine kinase activity with time showed that this increase was not ascribable to myocardial disease. We believe that the high proportion of CK-MB, confirmed by a comb...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 1997
ISSN: 0887-8013,1098-2825
DOI: 10.1002/(sici)1098-2825(1997)11:3<163::aid-jcla8>3.0.co;2-b