Immunodetection, Expression Strategy and Complementation of Turnip Crinkle Virus p28 and p88 Replication Components
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چکیده
منابع مشابه
A cis-replication element functions in both orientations to enhance replication of Turnip crinkle virus.
Turnip crinkle virus (TCV) (family Tombusviridae, genus Carmovirus) is a positive-sense RNA virus containing a 4054-base genome. Previous results indicated that insertion of Hairpin 4 (H4) into a TCV-associated satellite RNA enhanced replication 6-fold in vivo (Nagy, P., Pogany, J., Simon, A. E., 1999. EMBO J. 18:5653-5665). A detailed structural and functional analysis of H4 has now been perfo...
متن کاملA local, interactive network of 3' RNA elements supports translation and replication of Turnip crinkle virus.
The majority of the 3' untranslated region (UTR) of Turnip crinkle virus (TCV) was previously identified as forming a highly interactive structure with a ribosome-binding tRNA-shaped structure (TSS) acting as a scaffold and undergoing a widespread conformational shift upon binding to RNA-dependent RNA polymerase (RdRp). Tertiary interactions in the region were explored by identifying two highly...
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Proteolytic dissection of turnip crinkle virus subunit in solution.
Turnip crinkle virus (TCV) can be dissociated under mild conditions, yielding dimers of the protein subunit, as shown by chemical cross-linking and by gel filtration. These dimers have defined regions of proteolytic sensitivity, correDeterminat ion of the complete three-dimensional structure of tomato bushy stunt virus (TBSV) has revealed a number of remarkable features with implications for me...
متن کاملA multifunctional turnip crinkle virus replication enhancer revealed by in vivo functional SELEX.
The motif1-hairpin (M1H), located on (-)-strands of Turnip Crinkle Virus (TCV)-associated satellite RNA C (satC), is a replication enhancer and recombination hotspot. Results of in vivo genetic selection (SELEX: systematic evolution of ligands by exponential enrichment), where 28 bases of the M1H were randomized and then subjected to selection in plants, revealed that most winners contained one...
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ژورنال
عنوان ژورنال: Virology
سال: 1995
ISSN: 0042-6822
DOI: 10.1006/viro.1995.1434