Impact of asymmetrical flow field-flow fractionation on protein aggregates stability

Miniaturization of frit inlet asymmetrical flow field-flow fractionation.

A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of sample materials. The end effect contribut...

Effect of asymmetrical flow field-flow fractionation channel geometry on separation efficiency.

The separation efficiencies of three different asymmetrical flow field-flow fractionation (AF4) channel designs were evaluated using polystyrene latex standards. Channel breadth was held constant for one channel (rectangular profile), and was reduced either linearly (trapezoidal profile) or exponentially (exponential profile) along the length for the other two. The effective void volumes of the...

Stopless flow injection in asymmetrical flow field-flow fractionation using a frit inlet.

Stopless flow operation of asymmetrical flow field-flow fractionation (FFF) has been achieved by introducing a hydrodynamic relaxation method using a frit inlet. By using frit inlet injection, a focusing process which has been an essential part of runs at the asymmetrical flow FFF system can be completely avoided. Band broadening of an initial sample zone during hydrodynamic relaxation is discu...

Determination of Hamaker constants of polymeric nanoparticles by asymmetrical flow field-flow fractionation

The existing interaction forces between all objects are either of repulsive or attractive nature. Concerning attractive interactions, the determination of dispersion forces are of special interest since they appear in all colloidal systems and have a crucial influence on the properties and processes in these systems. The dispersion forces relate to the polarizability of the substances and can b...

Asymmetrical Flow Field-Flow-Fractionation in Pharmaceutical Analytics - Investigations in Aggregation Tendencies of Pharmaceutical Antibodies Dissertation