Imparting Nonnative Gold Binding Functionality by Bioengineering Bacterial Fimbrial TIP Protein FimH with Gold Binding Peptides
نویسندگان
چکیده
منابع مشابه
Inhibition of selectin binding by colloidal gold with functionalized shells.
Gold colloids with terminally functionalized sulfated thiol shells were found to bind to P- and L-selectins with IC(50) values in the picomolar range; branched acyclic epitopes show the highest affinity, whereas a sulfated carbohydrate mimetic provides the best selectivity.
متن کاملGold binding to red blood cells.
very much higher concentrations.1-4 We have also studied the distribution of gold in blood of patients with rheumatoid arthritis during treatment with sodium aurothiomalate and confirmed these results on the intersubject differences in distribution of gold. We surveyed the patients to determine factors which might affect the distribution of gold. From this survey we have identified smoking as t...
متن کاملHeterobinary adhesins based on the Escherichia coli FimH fimbrial protein.
The FimH adhesin of Escherichia coli type 1 fimbriae confers the ability to bind to D-mannosides by virtue of a receptor-binding domain located in its N-terminal region. This protein was engineered into a heterobifunctional adhesin by introducing a secondary binding site in the C-terminal region. The insertion of histidine clusters into this site resulted in coordination of various metal ions b...
متن کاملGold Binding by Native and Chemically Modified Hops Biomasses
Heavy metals from mining, smelting operations and other industrial processing facilities pollute wastewaters worldwide. Extraction of metals from industrial effluents has been widely studied due to the economic advantages and the relative ease of technical implementation. Consequently, the search for new and improved methodologies for the recovery of gold has increased. In this particular resea...
متن کاملHow GroES regulates binding of nonnative protein to GroEL.
At present, it is still enigmatic how the reaction cycle by which the Escherichia coli GroE chaperones mediate protein folding in the cell is coordinated with respect to the sequential order of binding and release of GroES, nucleotide, and nonnative protein. It is generally assumed that the asymmetric GroEL.GroES complex is the acceptor state for substrate protein. Nevertheless, this species is...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2018
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.11.1069