Improved Cell Line IPEC-J2, Characterized as a Model for Porcine Jejunal Epithelium

Improved Cell Line IPEC-J2, Characterized as a Model for Porcine Jejunal Epithelium

Cell lines matching the source epithelium are indispensable for investigating porcine intestinal transport and barrier properties on a subcellular or molecular level and furthermore help to reduce animal usage. The porcine jejunal cell line IPEC-J2 is established as an in vitro model for porcine infection studies but exhibits atypically high transepithelial resistances (TER) and only low active...

Polymethoxyflavone Apigenin-Trimethylether Suppresses LPS-Induced Inflammatory Response in Nontransformed Porcine Intestinal Cell Line IPEC-J2

The in vitro anti-inflammatory effect of apigenin and its trimethylated analogue (apigenin-trimethylether) has been investigated in order to evaluate whether these flavonoids could attenuate LPS-induced inflammation in IPEC-J2 non-transformed intestinal epithelial cells. Levels of IL-6, IL-8, TNF-α, and COX-2 mRNA were measured as a marker of inflammatory response. The extracellular H2O2 level ...

The profiles of mitochondrial respiration and glycolysis using extracellular flux analysis in porcine enterocyte IPEC-J2

Physiological Concentration of Exogenous Lactate Reduces Antimycin A Triggered Oxidative Stress in Intestinal Epithelial Cell Line IPEC-1 and IPEC-J2 In Vitro

Weaning triggers an adaptation of the gut function including luminal lactate generation by lactobacilli, depending on gastrointestinal site. We hypothesized that both lactobacilli and lactate influence porcine intestinal epithelial cells. In vivo experiments showed that concentration of lactate was significantly higher in gastric, duodenal and jejunal chyme of suckling piglets compared to their...

Effects of Crystalline Lactose on Expression of Sodium-dependent Glucose Transporter (SGLT)-1 mRNA

Supplementation of crystalline lactose increases SGLT-1 mRNA relative abundance, glucose transport to the basolateral compartment, and reduces the LPS-mediated inhibitory effect on glucose transport in IPEC-J2 cells. 1 Summary A preliminary experiment was conducted to evaluate the effects of crystalline lactose on the relative abundance of sodium-dependent glucose transporter (SGLT)-1 mRNA in v...