IMPROVED RESOLUTION IN LIGHT MICROSCOPE RADIOAUTOGRAPHY
نویسندگان
چکیده
منابع مشابه
Improved Resolution in Light Microscope Radioautography
Photographic presentat ion of l ight microscope rad ioautograms is difficult since the silver grains and the sections lie in two different planes. At the h igh magnificat ions necessary for m a x i m u m resolution of fine grain emulsions, it is impossible to get bo th the grains and the section in focus. This causes little difficulty under the microscope since one can focus at will on each pla...
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Experimental resolution values, half distances (HD), were determined for electron microscope radioautography with (14)C as the source of radioactivity. These were about a factor of 1.5-2 times higher than for tritium. Grain distributions normalized in units of HD were found to fit the "universal" curves previously obtained for tritium.
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A calibration method is described for measuring absolute radioautographic sensitivities under various experimental conditions. Sensitivities to (3)H and (35)S radiation, i.e. ratio of developed grains to radioactive decays in the specimen, were determined with Ilford L4 and Kodak NTE emulsions. The highest values obtained in monolayers of emulsion were (1/8) for (3)H and 1/21 for (35)S. The inf...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1967
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.33.3.728