Improving confidence in environmental DNA species detection
نویسندگان
چکیده
منابع مشابه
Species detection using environmental DNA from water samples.
The assessment of species distribution is a first critical phase of biodiversity studies and is necessary to many disciplines such as biogeography, conservation biology and ecology. However, several species are difficult to detect, especially during particular time periods or developmental stages, potentially biasing study outcomes. Here we present a novel approach, based on the limited persist...
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Effective management of rare species, including endangered native species and recently introduced nonindigenous species, requires the detection of populations at low density. For endangered species, detecting the localized distribution makes it possible to identify and protect critical habitat to enhance survival or reproductive success. Similarly, early detection of an incipient invasion by a ...
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The foundation for any ecological study and for the effective management of biodiversity in natural systems requires knowing what species are present in an ecosystem. We assessed fish communities in a stream using two methods, depletion-based electrofishing and environmental DNA metabarcoding (eDNA) from water samples, to test the hypothesis that eDNA provides an alternative means of determinin...
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Assignment of individuals to correct species or population of origin based on a comparison of allele profiles has in recent years become more accurate due to improvements in DNA marker technology. A method of assessing the error in such assignment problems is presented. The method is based on the exact hypergeometric distributions of contingency tables conditioned on marginal totals. The result...
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Environmental DNA (eDNA) is being rapidly adopted as a tool to detect rare animals. Quantitative PCR (qPCR) using probe-based chemistries may represent a particularly powerful tool because of the method's sensitivity, specificity, and potential to quantify target DNA. However, there has been little work understanding the performance of these assays in the presence of closely related, sympatric ...
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ژورنال
عنوان ژورنال: Molecular Ecology Resources
سال: 2015
ISSN: 1755-098X
DOI: 10.1111/1755-0998.12377