Improving peptide fragmentation by N-terminal derivatization with high proton affinity
نویسندگان
چکیده
منابع مشابه
On tissue protein identification improvement by N-terminal peptide derivatization.
Identification of potential markers of a physiological stage (e.g., pathology) discovered using MALDI-MSI is an important step in the understanding of signaling pathways or for providing sets of diagnosis and prognosis markers for clinical applications. Classically, identification can be achieved by extraction from a piece of tissue and proteomics strategies. However, this induces loss of infor...
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While de novo peptide sequencing is essential in many situations, it remains a difficult task. This is because peptide fragmentation results in complicated and often incomplete product ion spectra. In a previous study, we demonstrated that N-terminal charge derivatization with 4-amidinobenzoic acid (Aba) resulted in improved peptide fragmentation under lowenergy CID conditions. However, even wi...
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Background: Acute respiratory distress (ARD) is a critical respiratory failure due to lung injury of neonates leading to the clinical appearance of poor lung compliance. The aimed of the study was to evaluate the diagnostic values in differentiating respiratory from heart diseases with using of N-terminal pro brain natriuretic peptide (NT-pro BNP) and Atrial natriuretic peptide(ANP) in neonates...
متن کاملSimplifying fragmentation patterns of multiply charged peptides by N-terminal derivatization and electron transfer collision activated dissociation.
N-terminal peptide derivatization strategies used in conjunction with tandem mass spectrometry to yield simplified fragmentation patterns have shown limited success for the de novo sequencing of multiply charged peptides, including those predominantly formed in LC-ESI-MS experiments. Significant proton mobilization occurs for multiply charged peptides upon collisional activation, resulting in t...
متن کاملA Novel Strategy for the Analysis of Phosphopeptides, Coupling N-terminal Peptide Derivatization and Hplc Separation with Mass Spectrometry
• Here we present an N-terminal derivatization approach, using TMPP-Ac-Osu that improves the HPLC performance for small hydrophilic phosphopeptides. • This approach is compatible with LC/MS and LC/MALDI. • The chromatographic enhancement was evaluated by on-line nanoscale HPLC coupled with Electrospray on a Q-Tof PremierTM. • LC-MALDI/MS/MS analysis was also investigated. Both MS/MS on a Q-Tof ...
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ژورنال
عنوان ژورنال: Rapid Communications in Mass Spectrometry
سال: 2011
ISSN: 0951-4198
DOI: 10.1002/rcm.4962