In-Cell Protein Structures from 2D NMR Experiments

Part 2. Physical Basis of Protein Nmr Section 1. Theory of Fourier Transform Pulse Nmr Section 2. Nmr Experiments for Protein Studies Nmr Experiments for Sequential Resonance Assignments. Homonuclear 2d Section 3. Theories of Nmr Relaxation in Liquid

Nuclear magnetic resonance (NMR) spectroscopy, the detection of the Zeeman levels of nuclear spins in bulk condensed phase by resonant method through electromagnetic means, was pioneered by two research groups headed by Felix Bloch (Bloch et al., 1946a) and Edward M. Purcell (Purcell et al., 1946), respectively. It has since become an important experimental tool for physicists, chemists and mor...

Implementing ultrafast 2D NMR experiments on a Bruker Avance Spectrometer

Ultrafast solid-state 2D NMR experiments via orientational encoding.

Among the methods proposed in recent years toward the acceleration of multidimensional NMR acquisitions is an "ultrafast" approach, capable of delivering arbitrary 2D correlations within a single scan. This scheme operates by parallelizing the indirect-domain temporal incrementation involved in 2D acquisitions, using as aid an ancillary inhomogeneous frequency broadening acting in combination w...

1-D and 2D-NMR assignments of nigricin from Iris imbricata

Ethanolic crude extract of I. imbricata Lindl. (Iridaceae) was subjected to column chromatography on silica gel with varying portions of MeOH: CHCl3. Nigicin (irisolone) was isolated and its identification carried out by IR, UV, MS, 1-D and 2-D NMR spectroscopy.

Protein NMR Structures Refined without NOE Data

The refinement of low-quality structures is an important challenge in protein structure prediction. Many studies have been conducted on protein structure refinement; the refinement of structures derived from NMR spectroscopy has been especially intensively studied. In this study, we generated flat-bottom distance potential instead of NOE data because NOE data have ambiguity and uncertainty. The...