In-Depth Genetic Diversity and Population Structure of Endangered Peruvian Amazon Rosewood Germplasm Using Genotyping by Sequencing (GBS) Technology

Research studies on conservative genetics of endangered plants are very important to establish the management plans for conservation biodiversity. Rosewood is an evergreen tree Amazon region and its essential oil has great acceptance in medical cosmetic industry. The present study aimed explore genetic diversity population structure 90 rosewood accessions collected from eight localities Peruvian territory through DArTseq markers. A total 7485 informative markers resulted genotyping by sequencing (GBS) analysis were used molecular characterization germplasm. Mean values various calculated parameters like observed number alleles (1.962), effective (1.669), unbiased expected heterozygosity (0.411), percent polymorphism (93.51%) over entire germplasm showed existence a good level variations. Our results that Mairiricay was more diverse compared rest populations. Tamshiyacu-2 Mairiricay-15 found genetically distinct accessions. variance (AMOVA) reflected maximum variations (75%) due differences within implemented clustering algorithms, i.e., STRUCTURE, neighbor-joining principal coordinate (PCoA) separated studied basis their geographical locations. Diversity indices STRUCTURE-based populations subpopulation than populations, such reason, individuals belonging this should be reintroduction or reinforcement conservation. We envisage with will provide platform conservation, restoration upcoming years.