In-Vivo In-Vitro Screening of Ocimum basilicum L. Ecotypes with Differential UV-B Radiation Sensitivity

Elevated UV-B radiation (UV-B) has been previously reported to affect plant development, physiology, and promote the biosynthesis of UV-absorbing compounds. Sweet basil (Ocimum basilicum L.) is an aromatic herb, widely cultivated worldwide for its use in food, pharmaceuticals, cosmetics industry. This species exhibits high diversity among different ecotypes based on their geographical locations. There little research intra-specific photosynthetic metabolic differences tolerance across from areas. study evaluated protection responses nine O. accessions with geographic origins. Specifically, changes chlorophyll a fluorescence parameters leaf rosmarinic acid (RA) compound were assessed using “in vivo-vitro system” closed-type production system. Our results revealed significant variation mechanisms when plants treated doses. The accumulation RA increased significantly by light treatment OCI142, OCI148, OCI30, OCI160, OCI102, highest concentration measured OCI160 plants. ecotype showed value Fv/Fm ratio, 0.70, after 48 h. Recovery functionality was more rapid than other sweet accessions, which may indicate better capacity associated enhanced UV absorbing shows that represents effective mechanism reduce photoinhibitory photooxidative damage caused stress.