Increased Vδ1γδT cells predominantly contributed to IL-17 production in the development of adult human post-infectious irritable bowel syndrome

Abstract Background γδT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that γδ T producing IL-17 were involved pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile this kind disease remains unclear. Thus current study’s aim is to investigate functionally predominant its PI-IBS. Methods The total collected from peripheral blood patients with proportion Vδ1 Vδ2 was detected by FACS after stained anti δ1-PE δ2-APC. local colonic two subsets measured under laser confocal fluorescence microscope. enriched minoantibody-immuno-microbeads (MACS) method cultured, evaluated CCK-8 assay (proliferation), CD69/CD62L molecules expression (activation) ELISA (IL-17 production) respectively. Results 1. significantly increased while remained unchanged both tissue PI-IBS ( p < 0.05). 2. When cultured vitro, remarkably proliferated, activated produced Conclusions results suggest intestinal tissue, major