Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top-Down Mass Spectrometry

Ion Mobility Mass Spectrometry

for more complex analysers for automated sample processing and plant control. The separation time will continue to decrease; in the past there has been limited interest in fast separations but this could change as automated sample processing is developed. Increasing use of coupled techniques such as GC-GC, liquid chromatography-GC, and supercritical Suid chromatography-GC for the separation of ...

Ion Balance Mass Spectrometry

We have developed a technique to simultaneously compare the cyclotron frequencies of two single ions to obtain atomic mass ratios with fractional accuracies at or below 10−11. Much like a balance scale, this two-ion technique cancels many sources of noise and error – chief among them magnetic field noise. The new mass comparisons are used in combination with γ-ray wavelength measurements, perfo...

Ion Mobility-Mass Spectrometry

Comprehensive analysis of protein modifications by top-down mass spectrometry.

Mass spectrometry (MS)-based proteomics is playing an increasingly important role in cardiovascular research. Proteomics includes identification and quantification of proteins and the characterization of protein modifications, such as posttranslational modifications and sequence variants. The conventional bottom-up approach, involving proteolytic digestion of proteins into small peptides before...

Characterization of Modified RNA by Top-Down Mass Spectrometry**

Characteristic mass differences between fragment ions from backbone cleavage of RNA by electron detachment (d, w) and fragment ions from collisionally activated dissociation (c, y) provide extensive sequence information. Structure analysis by this approach should be especially useful for the detailed characterization of synthetic or post-transcriptionally modified RNA.