Infectious in vitro RNA transcripts derived from cloned cDNA of the cucurbit potyvirus, zucchini yellow mosaic virus

Construction of in vitro and in vivo infectious transcripts of a Taiwan strain of Zucchini yellow mosaic virus

The full-length cDNA of a Taiwan strain of Zucchini yellow mosaic virus (ZYMV TW-TN3) was constructed from five overlapping cDNA clones downstream from the bacteriophage T7 promoter in plasmid pT7ZYMV25. The plasmid was able to generate an in vitro transcript corresponding to TW-TN3 (9591 nt) with one extra guanosine residue at the 5 ́ terminus and a poly(A)95 tract at the 3 ́ end. In addition, p...

Study of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars

characterization of the zucchini yellow mosaic virus from squash in tehran province

zucchini yellow mosaic virus (zymv) is a potyvirus with a worldwide distribution. this virus causes serious economic losses in iran in many cucurbits. during 2002-2003, sam-ples were collected from squash fields in tehran province. five isolates (z1, z2, z3, z4 and z5) were inoculated on 27 species of cucurbitaceae, chenopodiaceae, amarantha-ceae,solanacae, leguminosae and ranunculaceae. chenop...

Inheritance of resistance to zucchini yellow mosaic virus and watermelon mosaic virus in watermelon.

High resistance to zucchini yellow mosaic virus-China strain (ZYMV-CH) and moderate resistance to watermelon mosaic virus (WMV) were found in a selection of PI 595203 (Citrullus lanatus var. lanatus), an Egusi type originally collected in Nigeria. Mixed inoculations showed primarily that these two viruses have no cross-protection. This fact may explain the high frequency of mixed infection ofte...

Generation of infectious pancreatic necrosis virus from cloned cDNA.

We developed a reverse genetics system for infectious pancreatic necrosis virus (IPNV), a prototype virus of the Birnaviridae family, with the use of plus-stranded RNA transcripts derived from cloned cDNA. Full-length cDNA clones of the IPNV genome that contained the entire coding and noncoding regions of RNA segments A and B were constructed. Segment A encodes a 106-kDa precursor protein which...