Infectivity of Viable but Non-Cultivable Cells of Pigmented and Nonpigmented Xanthomonas citri pv. anacardii Strains Demonstrate the Need to Establish Indexing Protocols for Cashew Propagules

Angular leaf spot of cashew tree in Brazil has been attributed to pigmented and nonpigmented strains Xanthomonas citri pv. anacardii. Due the possibility dissemination disease by propagating material, it is necessary understand survival mechanisms causal agent. Thus, present study aimed characterize behavior viable but non-cultivable cells (VBNC) two (CCRMTAQ13 CCRMTAQ18) one strain (IBSBF2579) X. anacardii, integrating silico, vitro, vivo studies. Thirteen genes associated with VBNC phase were identified genomes these strains. The log was observed at 24, 48, 120 h for CCRMTAQ13, CCRMTAQ18, IBSBF2579 strains, respectively. death 96 both 168 strain. Using qPCR analyses, possible occurrence three When inoculated, showed 100% incidence during phase, having longest incubation period (IP). did not differ concerning final severity (FS) phase. To our knowledge, this first report mechanism anacardii Furthermore, demonstrated that state potentially infective when they meet their host’s apoplast, which points need use integrated practices detect bacterium seedlings.