Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass® Scaffolds

Investigating the Vascularization of Tissue-Engineered Bone Constructs Using Dental Pulp Cells and 45S5 Bioglass® Scaffolds

Identification of a suitable cell source combined with an appropriate 3D scaffold is an essential prerequisite for successful engineering of skeletal tissues. Both osteogenesis and angiogenesis are key processes for bone regeneration. This study investigated the vascularization potential of a novel combination of human dental pulp stromal cells (HDPSCs) with 45S5 Bioglass® scaffolds for tissue-...

Bone tissue engineering using P-15 coated scaffolds and human dental pulp stromal cells

Introduction P-15 a fifteen residue synthetic peptide (GTPGPQGIAGQRGVV), is a structural analogue of the cell binding domain of Type 1 collagen [1]. P-15 adsorbed on anorganic bovine mineral (ABM-P15) scaffolds has been shown to enhance bone marrow stromal cell growth [2]. This study aimed to investigate the osteogenic potential of human dental pulp stromal cells (HDPSCs) in monolayer culture c...

The Use of Carbon Nanotubes to Reinforce 45S5 Bioglass-Based Scaffolds for Tissue Engineering Applications

Bioglass has been used for bone-filling material in bone tissue engineering, but its lean mechanical strength limits its applications in load-bearing positions. Carbon nanotubes (CNTs), with their high aspect ratio and excellent mechanical properties, have the potential to strengthen and toughen bioactive glass material without offsetting its bioactivity. Therefore, in this research, multiwall ...

Enhanced Stability of Calcium Sulfate Scaffolds with 45S5 Bioglass for Bone Repair

Calcium sulfate (CaSO₄), as a promising tissue repair material, has been applied widely due to its outstanding bioabsorbability and osteoconduction. However, fast disintegration, insufficient mechanical strength and poor bioactivity have limited its further application. In the study, CaSO₄ scaffolds fabricated by using selective laser sintering were improved by adding 45S5 bioglass. The 45S5 bi...

Functional integration of tissue-engineered bone constructs.