Investigation of Drug Resistant<i>Acinetobacter baumannii</i> Virulence Factors Implicated in Intracellular Survival Within Differentiated THP-1 Cells

Abstract Introduction/Objective Acinetobacter baumannii is a nosocomial opportunistic pathogen that usually associated with mucosal infections such as pneumonia, urinary tract or sepsis and less commonly more severe illnesses meningitis, wound infection, necrotizing fasciitis. Of late, A. baumanniihas been flagged by the CDC an urgent threat due to multidrug-resistant properties it has acquired, making major concern public health. Due sparsity of information on virulence factors immune evasion, difficult understand how this bacterium able gain foothold cause infections. We sought study fasciitis-associated strains interact human macrophages assess their capabilities in evasion host response. Methods/Case Report To end, extensively drug resistant was isolated at two timepoints (strains NFAb-1 NFAb-2) from patient over course lethal fasciitis infection led sepsis. The intracellular survival these compared each other, well type strains, ATCC 19606 17978, within macrophage-differentiated monocyte cell line, THP-1. Survival studies were complemented quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) investigate expression potential bacterial factors, Catalase E (KatE), Phospholipase C (PLC), Outer membrane protein A (ompA), Pilus (PilA), could contribute evasion. Results (if Case Study enter NA) assay demonstrated enhanced growth NFAb-2 17978. Examining gene expression, KatE, PLC, OmpA didn’t show any significant differences between while PilA had much higher Conclusion This data establishes nectrotizing fasciitis-causing are likely sruvivie macrophage-mediatied response pilus-assocated genes may play role interactions. our demonstrate need further mechanisms its ability evade hopes understanding combating