Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy
نویسندگان
چکیده
منابع مشابه
Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging.
We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detect...
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Using Monte-Carlo methods, we have investigated the signal-to-noise ratio obtainable for different fluorescence lifetime imaging methods. Quantum noise limited performance and mono-exponential decays were assumed. We have also investigated the importance of parameter choice and implementation for the different methods. In addition, our simulations were in many cases compared with analytical the...
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Measurement of endogenous free and bound NAD(P)H relative concentrations in living cells isa useful method for monitoring aspects of cellular metabolism, because the NADH∕NAD⁺ reduction-oxidation pair is crucial for electron transfer through the mitochondrial electron transport chain. Variations of free and bound NAD(P)H ratio are also implicated in cellular bioenergetic and biosynthetic metabo...
متن کاملTheoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy.
We investigate the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy, using both theoretical and Monte Carlo methods. Our analysis differs from previous work in that it incorporates the data fitting process used in real experiments, allows for the arbitrary choice of excitation and gain waveforms, and calculates lifetimes as well as associated F-values from higher h...
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ژورنال
عنوان ژورنال: Journal of the Optical Society of America A
سال: 2016
ISSN: 1084-7529,1520-8532
DOI: 10.1364/josaa.33.0000b1