Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
نویسندگان
چکیده
منابع مشابه
Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications.
The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure-property relationships that affect methods applying SG. These studies revealed the occurrence of intercalat...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 1362-4962
DOI: 10.1093/nar/gnh101