Isolation and Characterization of Tricarboxylic Acid Cycle Mutants of Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Sporulation of tricarboxylic acid cycle mutants of Bacillus subtilis.
A mutant of Bacillus subtilis 168 lacking aconitase (EC 4.2.1.3) was found to be blocked at stage 0 or I of sporulation. Although adenosine triphosphate levels, which normally decrease in tricarboxylic acid cycle mutants at the completion of exponential growth, could be maintained at higher levels by feeding metabolizable carbon sources, this did not permit the cells to progress further into th...
متن کاملDevelopmental block in citric acid cycle mutants of Bacillus subtilis.
Mutants deficient in different enzymes of the citric acid cycle can be subdivided into two groups according to the frequency at which they produce heat-resistant spores in nutrient sporulation medium. However, the majority of cells can develop in this medium only to the axial filament stage I of sporulation; aconitase and isocitrate dehydrogenase mutants need the addition of glutamate to reach ...
متن کاملIsolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملThe isolation and genetic characterization of mutants of the tryptophan system of Bacillus subtilis.
I T has previously been shown in this laboratory (CARLTON 1967) and elsewhere ( ANAGNOSTOPOULOS and CRAWFORD 1961 , 1967) that mutations leading to defects in tryptophan biosynthesis in Bacillus subtilis map in a cluster on the genome of this organism, and in an order which corresponds to that obtained for similar mutants in Escherichia coli (YANOFSKY and LENNOX 1959) and Salmonella typhimurium...
متن کاملMutants of Bacillus subtilis
Enzyme activities of glycolysis and glyconeogenesis are present in spores of Bacillus subtilis, the rate-limiting step of glucose (GLC) metabolism being its phosphorylation. GLC allows initiation of germination in the presence of fructose (FRU) and asparagine (ASN), not because it is used via the EmbdenMeyerhof path, but because it is oxidized in the nonphosphorylated form via the spore-specifi...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1971
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.106.3.848-855.1971