Isolation of extracellular vesicles with combined enrichment methods

Extracellular vesicles (EVs) are currently of tremendous interest in many research disciplines and EVs have potential for development EV diagnostics or therapeutics. Most well-known single isolation methods their particular advantages disadvantages terms purity yield. Combining provides additional to improve the efficacy both This review assesses contribution using combined by performing a two-step systematic literature analysis from all papers applying year 2019. resulted an overview various being applied isolations. A second database was generated studies within first that fairly compared multiple determining yield after isolation. From these databases it is shown most used not per definition best based on yield, indicating more factors play role choice which method choose than only method. included ~60% isolations were performed with methods. The majority differential ultracentrifugation alone combination ultrafiltration. When determined clearly outperformed methods, regardless type starting material used. recommended point would be use size-exclusion chromatography since this method, especially when low-speed centrifugation, highest purity, while still providing reasonable