Isolation of repeated and self-complementary sequences from E.coli DNA
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چکیده
منابع مشابه
Repeated DNA sequences in fungi.
Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10-20% repeated DNA sequences. There are approximately 100-110 copies of repeated DNA sequences of approximately 4 x 1...
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The nuclear DNA from all mouse strainsl-6 and mouse tissue" 2 thus far investigated exhibits two DNA bands when centrifuged to equilibrium in a CsCl density gradient. The principal band, which comprises 90 per cent of the nuclear DNA, is found at a buoyant density 0.01 gin cm-3 higher than the minor component, or satellite band, as it is generally called. Not only is this satellite DNA remarkab...
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The nuclear DNA of D. melanogaster contains DNA sequences that are repeated between ten and a hundred times more often than the next class of redundant DNA. This DNA, as a renatured duplex isolated on the basis of its renaturation kinetics, has a buoyant density of 1.691 (g/ml). In its native state it bands within the unique nuclear DNA peak (rho = 1.701). These sequences have been localized by...
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In an extensive analysis, using a range of restriction endonucleases, HinfI and TaqI were found to differentiate satellites I, II and III & IV. Satellite I is resistant to digestion by TaqI, but is cleaved by HinfI to yield three major fragments of approximate size 770, 850 and 950bp, associated in a single length of DNA. The 770bp fragment contains recognition sites for a number of other enzym...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1974
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/1.11.1539