Isoleucyl initiator tRNA does not initiate eucaryotic protein synthesis.
نویسندگان
چکیده
منابع مشابه
Editing mechanisms in protein synthesis. Rejection of valine by the isoleucyl-tRNA synthetase.
Although the isoleucyl-tRNA synthetase from Escherichia coli (IRS) does not catalyze the overall mischarging of tRNAIle with valine, it does undergo the first step of the reaction, the formation of an IRS-Val-AMP complex. The addition of tRNAIle to this complex leads to its quantitative hydrolysis and the IRS acts as an ATP pyrophosphate in the presence of valine and tRNAIle (Baldwin, A.N., and...
متن کاملHow many initiator tRNA genes does Escherichia coli need?
Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell appears to be unaffected by the removal of one copy. However, the fitness of ...
متن کاملFrom elongator tRNA to initiator tRNA.
We show that the two most important properties needed for a tRNA to function in initiation in Escherichia coli are its ability to be formylated and its ability to bind to the ribosomal P site. This conclusion is based on conversion of two different elongator tRNAs to ones that can act as initiators in E. coli. We transplanted the features unique to E. coli and eubacterial initiator tRNAs to E. ...
متن کاملInitiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA.
Protein synthesis in eukaryotic organelles such as mitochondria and chloroplasts is widely believed to require a formylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation. Here we show that initiation of protein synthesis in yeast mitochondria can occur without formylation of the initiator methionyl-tRNA (Met-tRNA(fMet)). The formylation reaction is catalyzed by methionyl-tRNA formyl...
متن کاملDependence of Mitochondrial Protein Synthesis Initiation on Formylation of the Initiator Methionyl-tRNA,
The effect of N’“-formyl-H,folate on mitochondrial peptide chain initiation has been studied in isolated mitochondria of Saccharomyces cerevisiae. The addition of N”‘-formyl-H,folate strongly stimulates the incorporation of amino acids into mitochondrial protein at both 6 and 15 mu Mg2+. Still higher stimulation (up to IO-fold) has been obtained in the production of de nouo synthesized initial ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)42903-6