Label-free multi-step microfluidic device for mechanical characterization of blood cells: Diabetes type II

The increasing interest to establish significant correlations between blood cell mechanical measurements and diseases, has led the promotion of microfluidic devices as attractive clinical tools for potential use in diagnosis. A multi-step device able separate red white cells (RBCs WBCs) from plasma simultaneously measure deformability (5 20% hematocrit) is presented this paper. employs passive separation based on cross-flow filtration principle, introduced at each daughter channel. At outlets, hyperbolic geometries allow single-cell analysis. was tested with five healthy fifteen diabetic type II voluntary donors. results have shown that WBCs lower than RBCs, no differences were observed pathological samples. In contrast, RBCs differences, exhibiting deformability. Shear rheology patients diabetes higher viscosity This demonstrated ability reduce concentration outlets down 1%, an ideal assessing deformability, under conditions. provide new insights quantitative information about hemodynamics vitro mellitus RBCs. Thus, such can be a promising complement diagnosis biological research part integrated blood-on-a-chip system.